reference mab atcc 19977 strain Search Results


electrocompetent atcc 19977  (ATCC)
99
ATCC electrocompetent atcc 19977
Electrocompetent Atcc 19977, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m abscessus complexa primary β lactam potentiator  (ATCC)
99
ATCC m abscessus complexa primary β lactam potentiator
M Abscessus Complexa Primary β Lactam Potentiator, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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illumina pe150 platform  (Novogene)
90
Novogene illumina pe150 platform
Illumina Pe150 Platform, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mab 062600 1635 1 6 50 50 50 50 mab 030804 1651 1 6 50 50 50 50 mab 010708 1655 1 6 50 50 50 50 open  (ATCC)
96
ATCC mab 062600 1635 1 6 50 50 50 50 mab 030804 1651 1 6 50 50 50 50 mab 010708 1655 1 6 50 50 50 50 open
Mab 062600 1635 1 6 50 50 50 50 Mab 030804 1651 1 6 50 50 50 50 Mab 010708 1655 1 6 50 50 50 50 Open, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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mab  (ATCC)
93
ATCC mab
NTM associated with NHBE cells at the air–liquid interface. (A and B) Infected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin <t>(red).</t> <t>MAC</t> and <t>MAB:</t> GFP (green). Cell nuclei: DAPI (blue). Extracellular (white arrows) and intracellular (yellow arrows) bacteria are shown across z-stacks. Scale bars: 5 μm. (A) Confocal images from a 57-year-old woman (57F). (B) Confocal images from a 75-year-old man (75M). MAC 100 and MAC 1,000: MAC at an MOI of 100 and 1,000; MAB 100 and MAB 1,000: MAB at an MOI of 100 and 1,000. (C) Mycobacteria growth in the Transwell at Days 1 and 3 after inoculation of 5 × 106 CFU (MOI = 100) or 5 × 107 CFU (MOI = 1,000) of MAC or MAB. The results are expressed as CFU per Transwell from three donors. *Significant difference between an MOI of 100 and an MOI of 1,000 at 1 day after infection (P < 0.05, two-way ANOVA followed by post hoc tests). Data are expressed as the mean ± SEM. (D) Percent of respiratory epithelial cells with associated bacteria. Tiled images of infected respiratory epithelium were analyzed and the percentage of infected cells was determined as the total number of cells associated with bacteria. Data are expressed as the mean ± SEM from four donors. No statistically significant changes were observed (two-way ANOVA followed by post hoc tests). CFU = colony-forming unit; MAB = Mycobacterium abscessus subsp. abscessus; MAC = Mycobacterium avium subsp. avium; MOI = multiplicity of infection; NHBE = normal primary human bronchial epithelial; NTM = nontuberculous mycobacteria.
Mab, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reference strain jcm 13569  (ATCC)
94
ATCC reference strain jcm 13569
NTM associated with NHBE cells at the air–liquid interface. (A and B) Infected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin <t>(red).</t> <t>MAC</t> and <t>MAB:</t> GFP (green). Cell nuclei: DAPI (blue). Extracellular (white arrows) and intracellular (yellow arrows) bacteria are shown across z-stacks. Scale bars: 5 μm. (A) Confocal images from a 57-year-old woman (57F). (B) Confocal images from a 75-year-old man (75M). MAC 100 and MAC 1,000: MAC at an MOI of 100 and 1,000; MAB 100 and MAB 1,000: MAB at an MOI of 100 and 1,000. (C) Mycobacteria growth in the Transwell at Days 1 and 3 after inoculation of 5 × 106 CFU (MOI = 100) or 5 × 107 CFU (MOI = 1,000) of MAC or MAB. The results are expressed as CFU per Transwell from three donors. *Significant difference between an MOI of 100 and an MOI of 1,000 at 1 day after infection (P < 0.05, two-way ANOVA followed by post hoc tests). Data are expressed as the mean ± SEM. (D) Percent of respiratory epithelial cells with associated bacteria. Tiled images of infected respiratory epithelium were analyzed and the percentage of infected cells was determined as the total number of cells associated with bacteria. Data are expressed as the mean ± SEM from four donors. No statistically significant changes were observed (two-way ANOVA followed by post hoc tests). CFU = colony-forming unit; MAB = Mycobacterium abscessus subsp. abscessus; MAC = Mycobacterium avium subsp. avium; MOI = multiplicity of infection; NHBE = normal primary human bronchial epithelial; NTM = nontuberculous mycobacteria.
Reference Strain Jcm 13569, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b1mcf9 b1mcf9 myca9 bacteria mab 2851c mycobacterium abscessus  (ATCC)
94
ATCC b1mcf9 b1mcf9 myca9 bacteria mab 2851c mycobacterium abscessus
NTM associated with NHBE cells at the air–liquid interface. (A and B) Infected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin <t>(red).</t> <t>MAC</t> and <t>MAB:</t> GFP (green). Cell nuclei: DAPI (blue). Extracellular (white arrows) and intracellular (yellow arrows) bacteria are shown across z-stacks. Scale bars: 5 μm. (A) Confocal images from a 57-year-old woman (57F). (B) Confocal images from a 75-year-old man (75M). MAC 100 and MAC 1,000: MAC at an MOI of 100 and 1,000; MAB 100 and MAB 1,000: MAB at an MOI of 100 and 1,000. (C) Mycobacteria growth in the Transwell at Days 1 and 3 after inoculation of 5 × 106 CFU (MOI = 100) or 5 × 107 CFU (MOI = 1,000) of MAC or MAB. The results are expressed as CFU per Transwell from three donors. *Significant difference between an MOI of 100 and an MOI of 1,000 at 1 day after infection (P < 0.05, two-way ANOVA followed by post hoc tests). Data are expressed as the mean ± SEM. (D) Percent of respiratory epithelial cells with associated bacteria. Tiled images of infected respiratory epithelium were analyzed and the percentage of infected cells was determined as the total number of cells associated with bacteria. Data are expressed as the mean ± SEM from four donors. No statistically significant changes were observed (two-way ANOVA followed by post hoc tests). CFU = colony-forming unit; MAB = Mycobacterium abscessus subsp. abscessus; MAC = Mycobacterium avium subsp. avium; MOI = multiplicity of infection; NHBE = normal primary human bronchial epithelial; NTM = nontuberculous mycobacteria.
B1mcf9 B1mcf9 Myca9 Bacteria Mab 2851c Mycobacterium Abscessus, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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pgex 4t1 vector  (Addgene inc)
93
Addgene inc pgex 4t1 vector
NTM associated with NHBE cells at the air–liquid interface. (A and B) Infected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin <t>(red).</t> <t>MAC</t> and <t>MAB:</t> GFP (green). Cell nuclei: DAPI (blue). Extracellular (white arrows) and intracellular (yellow arrows) bacteria are shown across z-stacks. Scale bars: 5 μm. (A) Confocal images from a 57-year-old woman (57F). (B) Confocal images from a 75-year-old man (75M). MAC 100 and MAC 1,000: MAC at an MOI of 100 and 1,000; MAB 100 and MAB 1,000: MAB at an MOI of 100 and 1,000. (C) Mycobacteria growth in the Transwell at Days 1 and 3 after inoculation of 5 × 106 CFU (MOI = 100) or 5 × 107 CFU (MOI = 1,000) of MAC or MAB. The results are expressed as CFU per Transwell from three donors. *Significant difference between an MOI of 100 and an MOI of 1,000 at 1 day after infection (P < 0.05, two-way ANOVA followed by post hoc tests). Data are expressed as the mean ± SEM. (D) Percent of respiratory epithelial cells with associated bacteria. Tiled images of infected respiratory epithelium were analyzed and the percentage of infected cells was determined as the total number of cells associated with bacteria. Data are expressed as the mean ± SEM from four donors. No statistically significant changes were observed (two-way ANOVA followed by post hoc tests). CFU = colony-forming unit; MAB = Mycobacterium abscessus subsp. abscessus; MAC = Mycobacterium avium subsp. avium; MOI = multiplicity of infection; NHBE = normal primary human bronchial epithelial; NTM = nontuberculous mycobacteria.
Pgex 4t1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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10 6 cfu/ml mab 19977  (MiddleBrook Pharmaceuticals)
90
MiddleBrook Pharmaceuticals 10 6 cfu/ml mab 19977
NTM associated with NHBE cells at the air–liquid interface. (A and B) Infected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin <t>(red).</t> <t>MAC</t> and <t>MAB:</t> GFP (green). Cell nuclei: DAPI (blue). Extracellular (white arrows) and intracellular (yellow arrows) bacteria are shown across z-stacks. Scale bars: 5 μm. (A) Confocal images from a 57-year-old woman (57F). (B) Confocal images from a 75-year-old man (75M). MAC 100 and MAC 1,000: MAC at an MOI of 100 and 1,000; MAB 100 and MAB 1,000: MAB at an MOI of 100 and 1,000. (C) Mycobacteria growth in the Transwell at Days 1 and 3 after inoculation of 5 × 106 CFU (MOI = 100) or 5 × 107 CFU (MOI = 1,000) of MAC or MAB. The results are expressed as CFU per Transwell from three donors. *Significant difference between an MOI of 100 and an MOI of 1,000 at 1 day after infection (P < 0.05, two-way ANOVA followed by post hoc tests). Data are expressed as the mean ± SEM. (D) Percent of respiratory epithelial cells with associated bacteria. Tiled images of infected respiratory epithelium were analyzed and the percentage of infected cells was determined as the total number of cells associated with bacteria. Data are expressed as the mean ± SEM from four donors. No statistically significant changes were observed (two-way ANOVA followed by post hoc tests). CFU = colony-forming unit; MAB = Mycobacterium abscessus subsp. abscessus; MAC = Mycobacterium avium subsp. avium; MOI = multiplicity of infection; NHBE = normal primary human bronchial epithelial; NTM = nontuberculous mycobacteria.
10 6 Cfu/Ml Mab 19977, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m. abscessus isolate m9507  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare m. abscessus isolate m9507
NTM associated with NHBE cells at the air–liquid interface. (A and B) Infected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin <t>(red).</t> <t>MAC</t> and <t>MAB:</t> GFP (green). Cell nuclei: DAPI (blue). Extracellular (white arrows) and intracellular (yellow arrows) bacteria are shown across z-stacks. Scale bars: 5 μm. (A) Confocal images from a 57-year-old woman (57F). (B) Confocal images from a 75-year-old man (75M). MAC 100 and MAC 1,000: MAC at an MOI of 100 and 1,000; MAB 100 and MAB 1,000: MAB at an MOI of 100 and 1,000. (C) Mycobacteria growth in the Transwell at Days 1 and 3 after inoculation of 5 × 106 CFU (MOI = 100) or 5 × 107 CFU (MOI = 1,000) of MAC or MAB. The results are expressed as CFU per Transwell from three donors. *Significant difference between an MOI of 100 and an MOI of 1,000 at 1 day after infection (P < 0.05, two-way ANOVA followed by post hoc tests). Data are expressed as the mean ± SEM. (D) Percent of respiratory epithelial cells with associated bacteria. Tiled images of infected respiratory epithelium were analyzed and the percentage of infected cells was determined as the total number of cells associated with bacteria. Data are expressed as the mean ± SEM from four donors. No statistically significant changes were observed (two-way ANOVA followed by post hoc tests). CFU = colony-forming unit; MAB = Mycobacterium abscessus subsp. abscessus; MAC = Mycobacterium avium subsp. avium; MOI = multiplicity of infection; NHBE = normal primary human bronchial epithelial; NTM = nontuberculous mycobacteria.
M. Abscessus Isolate M9507, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antimybpc3 antibody  (Proteintech)
93
Proteintech antimybpc3 antibody
NTM associated with NHBE cells at the air–liquid interface. (A and B) Infected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin <t>(red).</t> <t>MAC</t> and <t>MAB:</t> GFP (green). Cell nuclei: DAPI (blue). Extracellular (white arrows) and intracellular (yellow arrows) bacteria are shown across z-stacks. Scale bars: 5 μm. (A) Confocal images from a 57-year-old woman (57F). (B) Confocal images from a 75-year-old man (75M). MAC 100 and MAC 1,000: MAC at an MOI of 100 and 1,000; MAB 100 and MAB 1,000: MAB at an MOI of 100 and 1,000. (C) Mycobacteria growth in the Transwell at Days 1 and 3 after inoculation of 5 × 106 CFU (MOI = 100) or 5 × 107 CFU (MOI = 1,000) of MAC or MAB. The results are expressed as CFU per Transwell from three donors. *Significant difference between an MOI of 100 and an MOI of 1,000 at 1 day after infection (P < 0.05, two-way ANOVA followed by post hoc tests). Data are expressed as the mean ± SEM. (D) Percent of respiratory epithelial cells with associated bacteria. Tiled images of infected respiratory epithelium were analyzed and the percentage of infected cells was determined as the total number of cells associated with bacteria. Data are expressed as the mean ± SEM from four donors. No statistically significant changes were observed (two-way ANOVA followed by post hoc tests). CFU = colony-forming unit; MAB = Mycobacterium abscessus subsp. abscessus; MAC = Mycobacterium avium subsp. avium; MOI = multiplicity of infection; NHBE = normal primary human bronchial epithelial; NTM = nontuberculous mycobacteria.
Antimybpc3 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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middlebrook 7h9  (Becton Dickinson)
90
Becton Dickinson middlebrook 7h9
NTM associated with NHBE cells at the air–liquid interface. (A and B) Infected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin <t>(red).</t> <t>MAC</t> and <t>MAB:</t> GFP (green). Cell nuclei: DAPI (blue). Extracellular (white arrows) and intracellular (yellow arrows) bacteria are shown across z-stacks. Scale bars: 5 μm. (A) Confocal images from a 57-year-old woman (57F). (B) Confocal images from a 75-year-old man (75M). MAC 100 and MAC 1,000: MAC at an MOI of 100 and 1,000; MAB 100 and MAB 1,000: MAB at an MOI of 100 and 1,000. (C) Mycobacteria growth in the Transwell at Days 1 and 3 after inoculation of 5 × 106 CFU (MOI = 100) or 5 × 107 CFU (MOI = 1,000) of MAC or MAB. The results are expressed as CFU per Transwell from three donors. *Significant difference between an MOI of 100 and an MOI of 1,000 at 1 day after infection (P < 0.05, two-way ANOVA followed by post hoc tests). Data are expressed as the mean ± SEM. (D) Percent of respiratory epithelial cells with associated bacteria. Tiled images of infected respiratory epithelium were analyzed and the percentage of infected cells was determined as the total number of cells associated with bacteria. Data are expressed as the mean ± SEM from four donors. No statistically significant changes were observed (two-way ANOVA followed by post hoc tests). CFU = colony-forming unit; MAB = Mycobacterium abscessus subsp. abscessus; MAC = Mycobacterium avium subsp. avium; MOI = multiplicity of infection; NHBE = normal primary human bronchial epithelial; NTM = nontuberculous mycobacteria.
Middlebrook 7h9, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


NTM associated with NHBE cells at the air–liquid interface. (A and B) Infected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin (red). MAC and MAB: GFP (green). Cell nuclei: DAPI (blue). Extracellular (white arrows) and intracellular (yellow arrows) bacteria are shown across z-stacks. Scale bars: 5 μm. (A) Confocal images from a 57-year-old woman (57F). (B) Confocal images from a 75-year-old man (75M). MAC 100 and MAC 1,000: MAC at an MOI of 100 and 1,000; MAB 100 and MAB 1,000: MAB at an MOI of 100 and 1,000. (C) Mycobacteria growth in the Transwell at Days 1 and 3 after inoculation of 5 × 106 CFU (MOI = 100) or 5 × 107 CFU (MOI = 1,000) of MAC or MAB. The results are expressed as CFU per Transwell from three donors. *Significant difference between an MOI of 100 and an MOI of 1,000 at 1 day after infection (P < 0.05, two-way ANOVA followed by post hoc tests). Data are expressed as the mean ± SEM. (D) Percent of respiratory epithelial cells with associated bacteria. Tiled images of infected respiratory epithelium were analyzed and the percentage of infected cells was determined as the total number of cells associated with bacteria. Data are expressed as the mean ± SEM from four donors. No statistically significant changes were observed (two-way ANOVA followed by post hoc tests). CFU = colony-forming unit; MAB = Mycobacterium abscessus subsp. abscessus; MAC = Mycobacterium avium subsp. avium; MOI = multiplicity of infection; NHBE = normal primary human bronchial epithelial; NTM = nontuberculous mycobacteria.

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Transcriptional Response of Respiratory Epithelium to Nontuberculous Mycobacteria

doi: 10.1165/rcmb.2017-0218OC

Figure Lengend Snippet: NTM associated with NHBE cells at the air–liquid interface. (A and B) Infected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin (red). MAC and MAB: GFP (green). Cell nuclei: DAPI (blue). Extracellular (white arrows) and intracellular (yellow arrows) bacteria are shown across z-stacks. Scale bars: 5 μm. (A) Confocal images from a 57-year-old woman (57F). (B) Confocal images from a 75-year-old man (75M). MAC 100 and MAC 1,000: MAC at an MOI of 100 and 1,000; MAB 100 and MAB 1,000: MAB at an MOI of 100 and 1,000. (C) Mycobacteria growth in the Transwell at Days 1 and 3 after inoculation of 5 × 106 CFU (MOI = 100) or 5 × 107 CFU (MOI = 1,000) of MAC or MAB. The results are expressed as CFU per Transwell from three donors. *Significant difference between an MOI of 100 and an MOI of 1,000 at 1 day after infection (P < 0.05, two-way ANOVA followed by post hoc tests). Data are expressed as the mean ± SEM. (D) Percent of respiratory epithelial cells with associated bacteria. Tiled images of infected respiratory epithelium were analyzed and the percentage of infected cells was determined as the total number of cells associated with bacteria. Data are expressed as the mean ± SEM from four donors. No statistically significant changes were observed (two-way ANOVA followed by post hoc tests). CFU = colony-forming unit; MAB = Mycobacterium abscessus subsp. abscessus; MAC = Mycobacterium avium subsp. avium; MOI = multiplicity of infection; NHBE = normal primary human bronchial epithelial; NTM = nontuberculous mycobacteria.

Article Snippet: Strains of MAC (ATCC 35717) and MAB (ATCC 19977) were used.

Techniques: Infection, Labeling, Confocal Microscopy, Immunostaining, Bacteria

The percentage of ciliated cells decreased in NTM-infected respiratory epithelium. (A) Representative images of NHBE cells derived from a 67-year-old woman (67F). Infected or uninfected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin (red). MAC and MAB: GFP (green). Scale bars: 100 μm. (B) Tiled images of infected or uninfected respiratory epithelium were analyzed and the percentage of ciliated cells was determined as the total number of cells high in β-tubulin staining. Data are expressed as the mean ± SEM (three different donors [67F, 44F, and 67M], two fields of view per each donor). *Significant difference between infected and uninfected epithelium at Days 1 and 3 after infection (P < 0.05, one-way ANOVA followed by post hoc tests).

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Transcriptional Response of Respiratory Epithelium to Nontuberculous Mycobacteria

doi: 10.1165/rcmb.2017-0218OC

Figure Lengend Snippet: The percentage of ciliated cells decreased in NTM-infected respiratory epithelium. (A) Representative images of NHBE cells derived from a 67-year-old woman (67F). Infected or uninfected NHBE cells were fixed with paraformaldehyde, immunofluorescently labeled, and analyzed by confocal microscopy. Immunostaining: F-actin (magenta), β-tubulin (red). MAC and MAB: GFP (green). Scale bars: 100 μm. (B) Tiled images of infected or uninfected respiratory epithelium were analyzed and the percentage of ciliated cells was determined as the total number of cells high in β-tubulin staining. Data are expressed as the mean ± SEM (three different donors [67F, 44F, and 67M], two fields of view per each donor). *Significant difference between infected and uninfected epithelium at Days 1 and 3 after infection (P < 0.05, one-way ANOVA followed by post hoc tests).

Article Snippet: Strains of MAC (ATCC 35717) and MAB (ATCC 19977) were used.

Techniques: Infection, Derivative Assay, Labeling, Confocal Microscopy, Immunostaining, Staining